biobakery2025

The Huttenhower Lab > biobakery2025
Workshop on Microbial Community Multi-omics using the bioBakery

We are excited to host a week-long workshop on microbial community metagenomics, multi-omics, and statistics using the bioBakery this summer in Boston, June 2-6. The course will provide attendees with both instruction and hands-on experience with bioinformatics for profiling the taxonomic structure (microbes and viruses) and function of microbial communities from high-throughput molecular data, alongside statistical methods for associating those profiles with host phenotypes or environmental properties. We will focus on analysis of shotgun metagenomes, metatranscriptomes, and untargeted metabolomic data using the bioBakery: a computing environment comprising reproducible methods for microbial community analysis. Participants will need to bring their own laptops and have some basic familiarity with working at the command line, but prior knowledge of our software or microbial community analysis is not needed.

The course will be hosted and delivered by members of the Huttenhower Lab and the Harvard Chan Microbiome in Public Health Center (HCMPH). Our philosophy is to train participants in the most relevant topics in microbial community analysis in a vibrant, immersive, and inclusive environment. We welcome all skill levels for this in-person course and look forward to hosting a productive and engaging workshop! See below for an overview of the course program, its details, and registration information. Please contact Kelsey Thompson (kthompson@hsph.harvard.edu) with any questions about the workshop.

When: June 2-6, 2025

Where: 300 Binney Street, Broad Institute, Cambridge, MA

Instructors: Drs. Curtis Huttenhower, Eric Franzosa, Kelsey Thompson, Jacob Nearing, and other members of the HCMPH.

 

Registration: Eventbrite     

 

 

Day 1 (9am-6pm)
Morning: 9am-12:30pm
Lecture: General introduction
Student introductions
Lecture: Intro. to microbial community multi'omics
Lecture: Experimental considerations in microbiome sequencing
Lab: Google Cloud setup
12:30pm-2pm Lunch
Afternoon: 2pm-6pm
Lab: Sequencing QC with KneadData
Lecture: Taxonomic profiling
Lab: Taxonomic profiling with MetaPhlAn
Lecture: The Human Microbiome Projects (HMP1+2)
Open discussion / questions / wrap-up
Course dinner - MEX (500 Technology Square, Cambridge)
Day 2 (9am-6pm)
Morning: 9am-12:30pm
BONUS Lab: Intro to command line
Lecture: Strain profiling
Lab: Strain profiling with StrainPhlAn
Lab: Tree viz with GraPhlAn
12:30pm-2pm Lunch
Afternoon: 2pm-6pm
Lecture: Viral profiling
Lab: Viral profiling with BAQLaVa
Lecture: Functional profiling
Lab: Functional profiling with HUMAnN
Open discussion / questions / wrap-up
End of day
Day 3 (9am-6pm)
Morning: 9am-12:30pm
BONUS LAB: Intro to R
Lecture: Metagenomic assembly and applications
Lab: Assembly and SGB creation
Lecture: SGBs and microbial phylogenetics
Lab: Microbial phylogenetics with PhyloPhlAn
12:30pm-2pm Lunch
Afternoon: 2pm-6pm
Lab: Prioritizing bioactive genes with MetaWIBELE
Lecture: Gene transfer in microbial communities
Lab: Detecting LGT with WAAFLE
Lab: Lecture: Meta'omic visualization
Open discussion / questions / wrap-up
End of day
Day 4 (9am-6pm)
Morning: 9am-12:30pm
BONUS LAB: Meta'omic viz in R
Lecture: Microbial community study design
Lecture: Statistics for microbial multi'omics
Lab: Strain epidemiologic testing with Anpan
Lecture: Metatranscriptomics (MTX)
12:30pm-2pm Lunch
Afternoon: 2pm-6pm
Lab: Microbial community statistics with MaAsLin
Lecture: Microbiome metabolomics
Lab: Prioritizing bioactive metabolites with MACARRoN
Open discussion / questions / wrap-up
End of day
Day 5 (9am-6pm)
Morning: 9am-12:30pm
BONUS LAB: High-dim. testing in R
Lecture: Methods for multi'omic data integration
Lab: Multi'omic integration with HAllA
Lecture: Meta-analysis with microbial community data
Lab: Meta-analysis with MMUPHin
12:30pm-2pm Lunch
Afternoon: 2pm-6pm
Lecture: Intro. to AnADAMA and bioBakery workflows
Lab: bioBakery workflows
Lecture: Reproducibility in microbiome studies (MBQC) - People's choice
Open discussion / questions / wrap-up
End of day
Course location and logistics

This course will be held at the new building for the Broad Institute in Cambridge, MA located at 300 Binney in Kendall Square.

Tuition to attend: $450  (not including room, board, or transportation to the event location)

Registration: Eventbrite

(To register to attend this event please use this link to complete registration through Eventbrite)

Nearby hotels list:

  • Boston Marriott Cambridge
  • The Kendall Hotel
  • Residence Inn Boston Cambridge
  • The Fairfield Inn and Suites Boston/Cambridge
  • Hampton Inn by Hilton Boston/Cambridge